Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods.
Int J Food Microbiol
; 118(3): 274-84, 2007 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-17822795
ABSTRACT
Development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. We established a simple typing method for Listeria monocytogenes using MLSSCP (Multilocus Single Strand Conformation Polymorphism) analysis. Four virulence genes, hlyA, iap, actA and inlB were amplified by PCR, digested with endonucleases and applied to gels for SSCP. As banding patterns have been shown to reflect even a single nucleotide difference, this method has a potential discriminatory power comparable to that of sequencing analysis. The 64 strains isolated from five meat processing plants were divided into 18 groups by this MLSSCP. Additionally, clustering obtained with this method showed strong correspondence with phylogenetic lineages I and II, and was achieved with much less expenditure in time and cost than is required for other methods, such as MLST. The validity of the MLSSCP lineage classification was confirmed by PFGE, AFLP and ribotyping results. This newly developed MLSSCP method is suitable when obtaining accurate results quickly and simply is crucial.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Contaminação de Alimentos
/
Polimorfismo Conformacional de Fita Simples
/
Fatores de Virulência
/
Listeria monocytogenes
/
Produtos da Carne
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Int J Food Microbiol
Assunto da revista:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão