Genomic differences between guinea pig lethal and nonlethal Marburg virus variants.
J Infect Dis
; 196 Suppl 2: S305-12, 2007 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-17940965
ABSTRACT
The complete genome sequences of 2 closely related plaque-derived variants of Marburg virus (MARV) species Lake Victoria marburgvirus, strain Musoke, indicate only a few regions of the RNA genome as underlying the differences between the 2 viruses. One variant is >90% lethal for guinea pigs and the other much less virulent, when guinea pigs are challenged with 1000 pfu of virus. Only 4 mutations that result in amino acid changes were identified, 1 in viral matrix protein VP40 and 3 in L, the RNA-dependent RNA polymerase. In addition, 6 differences were identified in noncoding regions of transcribed mRNA, and 1 silent codon change was identified in the L gene. Interestingly, the amino acid mutation identified in VP40 occurs in a nonconserved loop structure between 2 domains that are homologues only among MARV species. The L gene mutations were equally intriguing, clustering near a highly conserved motif in viral RNA-dependent RNA polymerases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Marburgvirus
/
Doença do Vírus de Marburg
Limite:
Animals
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos