Chemical screening of olive biophenol extracts by hyphenated liquid chromatography.
Anal Chim Acta
; 603(2): 176-89, 2007 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-17963838
ABSTRACT
Chemical screening using reversed phase HPLC-photodiode array detection (RPLC-DAD) and RPLC-electrospray ionisation mass spectrometry (RPLC-ESI-MS) is widely applied as an approach to streamline natural products research. The full potential of this approach is demonstrated in this paper by application to the chemical screening of olive products including olive mill waste (OMW). Out of 100 biophenols previously reported in olive products, the on-line RPLC-DAD-ESI-MS was able to confirm the presence of 52 compounds in OMW. This included a number of simple phenols, flavonoids and secoiridoids. By careful examination of the combined DAD and ESI-MS data, extra information was elucidated including the site of glycosidation on the phenol ring of hydroxytyrosol; the identity of the other luteolin-glucoside isomer as luteolin-4'-O-glucoside; identifying rutin rather than the previously reported hesperidin (and the reasons for possible mis-assignment); and the detection of diastereomers of 4-hydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (4-HPEA-DEDA) and 3,4-dihydroxyphenylethyl alcohol-deacetoxy elenolic acid dialdehyde (3,4-DHPEA-DEDA).
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas por Ionização por Electrospray
/
Olea
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Austrália