Re-engineering an in-vitro evolved ribozyme.
Nucleic Acids Symp Ser (Oxf)
; (51): 381-2, 2007.
Article
em En
| MEDLINE
| ID: mdl-18029746
ABSTRACT
Ribox02 is an in-vitro evolved RNA molecule that exhibits the activity analogous to that of alcohol dehydrogenase, catalyzing redox reactions in a cofactor dependent manner. It forms a compact pseudo-knotted structure and oxidizes an alcohol seven orders of magnitude faster than the estimated spontaneous rate. To gain insights into the structure-function relationship of Ribox02, we firstly performed mutational studies to identify the critical nucleotide bases for catalysis and then engineered the ribozyme structure to improve its overall fold. The re-engineered Ribox02, named Robust, was able to catalyze the reaction approximately 25% more efficiently, leading us to generate a transacting system.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Álcool Desidrogenase
/
RNA Catalítico
Idioma:
En
Revista:
Nucleic Acids Symp Ser (Oxf)
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos