Molecular code for transmembrane-helix recognition by the Sec61 translocon.
Nature
; 450(7172): 1026-30, 2007 Dec 13.
Article
em En
| MEDLINE
| ID: mdl-18075582
ABSTRACT
Transmembrane alpha-helices in integral membrane proteins are recognized co-translationally and inserted into the membrane of the endoplasmic reticulum by the Sec61 translocon. A full quantitative description of this phenomenon, linking amino acid sequence to membrane insertion efficiency, is still lacking. Here, using in vitro translation of a model protein in the presence of dog pancreas rough microsomes to analyse a large number of systematically designed hydrophobic segments, we present a quantitative analysis of the position-dependent contribution of all 20 amino acids to membrane insertion efficiency, as well as of the effects of transmembrane segment length and flanking amino acids. The emerging picture of translocon-mediated transmembrane helix assembly is simple, with the critical sequence characteristics mirroring the physical properties of the lipid bilayer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bicamadas Lipídicas
/
Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Nature
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Suécia