Bayesian inference for improved single molecule fluorescence tracking.
Biophys J
; 94(12): 4932-47, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18339757
ABSTRACT
Single molecule tracking is widely used to monitor the change in position of lipids and proteins in living cells. In many experiments in which molecules are tagged with a single or small number of fluorophores, the signal/noise ratio may be limiting, the number of molecules is not known, and fluorophore blinking and photobleaching can occur. All these factors make accurate tracking over long trajectories difficult and hence there is still a pressing need to develop better algorithms to extract the maximum information from a sequence of fluorescence images. We describe here a Bayesian-based inference approach, based on a trans-dimensional sequential Monte Carlo method that utilizes both the spatial and temporal information present in the image sequences. We show, using model data, where the real trajectory of the molecule is known, that our method allows accurate tracking of molecules over long trajectories even with low signal/noise ratio and in the presence of fluorescence blinking and photobleaching. The method is then applied to real experimental data.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biopolímeros
/
Reconhecimento Automatizado de Padrão
/
Inteligência Artificial
/
Interpretação de Imagem Assistida por Computador
/
Aumento da Imagem
/
Microscopia de Vídeo
/
Microscopia de Fluorescência
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
Biophys J
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Reino Unido