[All-trans retinoic acid enhances bystander effect of suicide-gene therapy against androgen-unresponsive prostate cancer].
Zhonghua Nan Ke Xue
; 14(2): 122-5, 2008 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-18390175
ABSTRACT
OBJECTIVE:
To investigate the enhancing effect of all-trans retinoic acid (ATRA) on the bystander effect of the herpes simplex virus thymidine kinase(HSV-TK)/ganciclovir (GCV) against androgen unresponsive prostate cancer.METHODS:
The bystander effect of the HSV-TK/GCV system was measured by methyl thiazolyl tetrazolium (MTT) assay on PC-3 cells before and after ATRA treatment. The growth and the histopathology of transplant tumors were observed in 4 groups of nude mice with prostate cancer.RESULTS:
ATRA augmented significantly the bystander effect of the HSV-TK/GCV system by reducing TK positive PC-3 cells from 50% to 30% (P < 0.05). HSV-TK showed an inhibiting effect, while ATRA with the HSV-TK/GCV system produced significant effect on prostate cancer 1 week earlier than the former (P < 0.05).CONCLUSION:
ATRA can argument the in vivo and in vitro bystander effect of the HSV-TK/GCV system in the treatment of androgen unresponsive prostate cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Tretinoína
/
Terapia Genética
/
Efeito Espectador
/
Genes Transgênicos Suicidas
Limite:
Animals
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China