Selective ablation of an astroglial subset by toxic gene expression driven by tenascin promoter.
Neurol Res
; 30(7): 701-9, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18489816
ABSTRACT
OBJECTIVE:
In vitro culture, one of the astroglia-derived extracellular matrix proteins, tenascin, expressed highly in fibrous astrocytes, whereas it expressed only low levels in protoplasmic astrocytes. We devised a method of selectively altering the population of astroglial subsets in primary culture of astrocytes derived from embryonic mouse brains using toxic gene expression driven by the tenascin promoter.METHODS:
We have identified that the segment of 512-bases of 5'-flanking plus 243-bases leader sequences of the mouse tenascin gene contains maximum promoter activity in primary culture of astrocytes by deletion analysis of 5'-upstream region. This promoter element was used to specifically express the herpes simplex virus thymidine kinase (HSV-TK) gene in tenascin-positive astrocytes.RESULTS:
This strategy allowed us to selectively decrease tenascin-positive astrocytes at the optimal concentration of ganciclovir, which is cytotoxic in HSV-TK-expressing cells.DISCUSSION:
This approach should be useful for examining the role of the tenascin-negative astroglial subset in the development and regeneration of the central nervous system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timidina Quinase
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Transfecção
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Astrócitos
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Regiões Promotoras Genéticas
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Tenascina
Limite:
Animals
Idioma:
En
Revista:
Neurol Res
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão