Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers.
Biotechniques
; 44(7): 924-6, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18533903
ABSTRACT
We have developed a modular method of plasmid construction that can join multiple DNA components in a single reaction. A nicking enzyme is used to create 5' and 3' overhangs on PCR-generated DNA components. Without the use of ligase or restriction enzymes, components are joined using oligonucleotide linkers that recognize the overhangs. By specifying the sequences of the linkers, desired components can be assembled in any combination and order to generate different plasmid vectors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Plasmídeos
/
Clonagem Molecular
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos