Autoantibodies against matrix metalloproteinase-1 in patients with localized scleroderma.
J Dermatol Sci
; 52(1): 47-54, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18565735
ABSTRACT
BACKGROUND:
Localized scleroderma (LSc) is characterized by cutaneous fibrosis and various autoantibodies.OBJECTIVE:
To determine the presence or levels of antibodies (Abs) against matrix metalloproteinase (MMP)-1 and their clinical relevance in LSc.METHODS:
Anti-MMP-1 Ab was examined by ELISA (Enzyme-Linked ImmunoSorbent Assay) and immunoblotting using human recombinant MMP-1. MMP-1 collagenase activity was determined using biotinylated collagen as substrate and the amount of cleaved biotinylated fragments of collagen by MMP-1 was measured by ELISA.RESULTS:
LSc patients exhibited significantly elevated IgG anti-MMP-1 Ab levels relative to normal controls at similar level of patients with systemic sclerosis (SSc). However, IgG anti-MMP-1 Ab levels were comparable among the 3 LSc subgroups morphea, linear scleroderma, and generalized morphea. When absorbance values higher than the mean+2S.D. of normal controls were considered positive, IgG or IgM anti-MMP-1 Ab was found in 46% and 49% of total LSc patients and SSc patients, respectively. Anti-MMP-1 Ab was detected most frequently in morphea patients (60%), followed by linear scleroderma patients (47%) and then generalized morphea patients (25%). LSc patients positive for IgG anti-MMP-1 Ab had elevated levels of IgG anti-single-stranded DNA Ab, IgG anti-nucleosome Ab, and shorter disease duration relative to those negative. The presence of anti-MMP-1 Ab in LSc patients was confirmed by immunoblotting. IgG isolated from LSc patients' sera positive for IgG anti-MMP-1 Ab by ELISA inhibited MMP-1 collagenase activity.CONCLUSION:
These results suggest that anti-MMP-1 autoantibody is a novel autoantibody in LSc.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esclerodermia Localizada
/
Autoanticorpos
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Metaloproteinase 1 da Matriz
Limite:
Adolescent
/
Adult
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Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
J Dermatol Sci
Assunto da revista:
DERMATOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão