Use of a Cry1Ac-resistant line of Helicoverpa armigera (Lepidoptera: Noctuidae) to detect novel insecticidal toxin genes in Bacillus thuringiensis.
Curr Microbiol
; 57(3): 175-80, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18592310
ABSTRACT
This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac (line ISOC) was used to screen Cry1Ac-expressing strains of B. thuringiensis for additional toxins with activity against H. armigera. Using this approach, a number of Cry1Ac-producing strains with significant toxicity toward Cry1Ac-resistant H. armigera were identified. When the insecticidal protein complement of one of these strains, C81, was examined in detail, a novel cry2 gene (cry2Af1) was detected.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus thuringiensis
/
Proteínas de Bactérias
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Resistência a Inseticidas
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Endotoxinas
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Proteínas Hemolisinas
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Lepidópteros
Limite:
Animals
Idioma:
En
Revista:
Curr Microbiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Austrália