Dual promoters control expression of the Bacillus anthracis virulence factor AtxA.
J Bacteriol
; 190(19): 6483-92, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18676674
ABSTRACT
The AtxA virulence regulator of Bacillus anthracis is required for toxin and capsule gene expression. AtxA is a phosphotransferase system regulatory domain-containing protein whose activity is regulated by phosphorylation/dephosphorylation of conserved histidine residues. Here we report that transcription of the atxA gene occurs from two independent promoters, P1 (previously described by Dai et al. [Z. Dai, J. C. Sirard, M. Mock, and T. M. Koehler, Mol. Microbiol. 161171-1181, 1995]) and P2, whose transcription start sites are separated by 650 bp. Both promoters have -10 and -35 consensus sequences compatible with recognition by sigma(A)-containing RNA polymerase, and neither promoter depends on the sporulation sigma factor SigH. The dual promoter activity and the extended untranslated mRNA suggest that as-yet-unknown regulatory mechanisms may act on this region to influence the level of AtxA in the cell.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus anthracis
/
Proteínas de Bactérias
/
Transativadores
/
Regiões Promotoras Genéticas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos