[Molecular epidemiology of enterohaemorrhagic Esacherichia coli O157 in some areas in China].
Zhonghua Liu Xing Bing Xue Za Zhi
; 29(1): 55-8, 2008 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-18785480
ABSTRACT
OBJECTIVE:
To understand the epidemiological characteristics of enterohaemorrhagic Escherichia coli (EHEC) O157 and to determine the degree of its genetic relations.METHODS:
Polymerase chain reaction (PCR) techniques and chromosomal DNA digested by restriction enzyme Xba I according to PulseNet directions by pulsed field gel electrophoresis (PFGE) method were applied to 300 E. coli O157 strains isolated from patients and animal sources from 1988 to 2005 from Henan, Jiangsu and Anhui provinces.RESULTS:
Very high prevalence of stx2 gene in EHEC O157H7 strains isolated from some provinces of China was found and variation existed in some strains. We got 161 PFGE patterns from 300 strains. The stx2-producing strains could be clearly separated from stx2 variation-producing strains.CONCLUSION:
The variability of restriction enzyme-digestion patterns of O157 genomes suggested that the presence of some genomic diversity among the strains did exist.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epidemiologia Molecular
/
Escherichia coli O157
Tipo de estudo:
Risk_factors_studies
/
Screening_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Liu Xing Bing Xue Za Zhi
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China