Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia.
Mem Inst Oswaldo Cruz
; 103(5): 493-6, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18797765
ABSTRACT
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3alpha gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 alpha gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 %). The Pvmsp-3alpha gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium vivax
/
Polimorfismo Genético
/
DNA de Protozoário
Limite:
Animals
/
Humans
País/Região como assunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Colômbia