Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh.
Microbiol Immunol
; 52(10): 508-11, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18822085
ABSTRACT
The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 microg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tetraciclina
/
Resistência a Tetraciclina
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Antibacterianos
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Microbiol Immunol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Bangladesh