Localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively.
EMBO J
; 27(22): 3011-23, 2008 Nov 19.
Article
em En
| MEDLINE
| ID: mdl-18923417
The Smc5/6 holocomplex executes key functions in genome maintenance that include ensuring the faithful segregation of chromosomes at mitosis and facilitating critical DNA repair pathways. Smc5/6 is essential for viability and therefore, dissecting its chromosome segregation and DNA repair roles has been challenging. We have identified distinct epigenetic and post-translational modifications that delineate roles for fission yeast Smc5/6 in centromere function, versus replication fork-associated DNA repair. We monitored Smc5/6 subnuclear and genomic localization in response to different replicative stresses, using fluorescence microscopy and chromatin immunoprecipitation (ChIP)-on-chip methods. Following hydroxyurea treatment, and during an unperturbed S phase, Smc5/6 is transiently enriched at the heterochromatic outer repeats of centromeres in an H3-K9 methylation-dependent manner. In contrast, methyl methanesulphonate treatment induces the accumulation of Smc5/6 at subtelomeres, in an Nse2 SUMO ligase-dependent, but H3-K9 methylation-independent manner. Finally, we determine that Smc5/6 loads at all genomic tDNAs, a phenomenon that requires intact consensus TFIIIC-binding sites in the tDNAs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Cromossômicas não Histona
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Heterocromatina
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Centrômero
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Telômero
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Proteínas de Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
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Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos