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Localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively.
Pebernard, Stephanie; Schaffer, Lana; Campbell, Daniel; Head, Steven R; Boddy, Michael N.
Afiliação
  • Pebernard S; Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
EMBO J ; 27(22): 3011-23, 2008 Nov 19.
Article em En | MEDLINE | ID: mdl-18923417
The Smc5/6 holocomplex executes key functions in genome maintenance that include ensuring the faithful segregation of chromosomes at mitosis and facilitating critical DNA repair pathways. Smc5/6 is essential for viability and therefore, dissecting its chromosome segregation and DNA repair roles has been challenging. We have identified distinct epigenetic and post-translational modifications that delineate roles for fission yeast Smc5/6 in centromere function, versus replication fork-associated DNA repair. We monitored Smc5/6 subnuclear and genomic localization in response to different replicative stresses, using fluorescence microscopy and chromatin immunoprecipitation (ChIP)-on-chip methods. Following hydroxyurea treatment, and during an unperturbed S phase, Smc5/6 is transiently enriched at the heterochromatic outer repeats of centromeres in an H3-K9 methylation-dependent manner. In contrast, methyl methanesulphonate treatment induces the accumulation of Smc5/6 at subtelomeres, in an Nse2 SUMO ligase-dependent, but H3-K9 methylation-independent manner. Finally, we determine that Smc5/6 loads at all genomic tDNAs, a phenomenon that requires intact consensus TFIIIC-binding sites in the tDNAs.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Cromossômicas não Histona / Heterocromatina / Centrômero / Telômero / Proteínas de Ciclo Celular / Proteínas de Schizosaccharomyces pombe / Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina Idioma: En Revista: EMBO J Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Cromossômicas não Histona / Heterocromatina / Centrômero / Telômero / Proteínas de Ciclo Celular / Proteínas de Schizosaccharomyces pombe / Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina Idioma: En Revista: EMBO J Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Estados Unidos