Endogenous immunoglobulin expression in mu transgenic mice.
Int Immunol
; 3(2): 185-96, 1991 Feb.
Article
em En
| MEDLINE
| ID: mdl-1902746
ABSTRACT
Transgenic mice (M54) containing a functional mu heavy chain were examined to determine the effects of the transgene on rearrangement and expression of endogenous immunoglobulin genes. Two major novel findings are presented. (i) In transgenic mice, the expressed endogenous VH repertoire in LPS-generated B cell blasts and hybridomas is skewed toward expression of JH-proximal VH families (VH7183 and Q52). (ii) There is an increase in the frequency of B cells expressing lambda light chain genes in transgenic mice. Furthermore, in Abelson-MuLV transformed pre-B cells, VH to DJH is inhibited more than the D to JH rearrangement. The results presented indicate that the transgene skews the expressed VH repertoire by inhibiting the VH to DJH rearrangement while permitting an expansion of B cells expressing limited VH and lambda light chain genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Imunoglobulinas
/
Cadeias mu de Imunoglobulina
Limite:
Animals
Idioma:
En
Revista:
Int Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Marrocos