A rodent model of metabolic surgery for study of type 2 diabetes and positron emission tomography scanning of beta cell mass.
Surg Obes Relat Dis
; 5(2): 212-7, 2009.
Article
em En
| MEDLINE
| ID: mdl-19136315
ABSTRACT
BACKGROUND:
Type 2 diabetes mellitus is a worldwide healthcare problem with major socioeconomic implications. Metabolic surgical procedures have been shown to improve diabetes, but the mechanism of action is poorly understood. The Goto-Kakizaki (GK) rodent is a type 2 diabetic animal model that is ideally situated for studying the effect of surgery on diabetes; however, the operative mortality is high. The aim of this study was to describe the operative technique, improvements in perioperative management, and the technique of micro-positron emission tomography (PET) scanning of the beta-cell mass in GK rodents.METHODS:
A total of 53 GK rats were divided into 1 of 3 operative groups sham, sleeve gastrectomy, and duodenojejunal bypass. A subset of animals underwent micro-PET scanning with [11C]-dihydrotetrabenazine to determine the vesicular monoamine transporter 2 binding index, an indicator of beta-cell mass.RESULTS:
The 30-day mortality in the sham and sleeve gastrectomy rodents was 0; however, 2 sleeve gastrectomy rodents developed enterocutaneous fistula and 1 developed an abscess. In the duodenojejunal bypass group, the initial mortality rate was close to 90%; however, refinements in the surgical technique and perioperative management (fluids, antibiotics, pain control) lowered the mortality rate to 60%. The surgical technique is discussed in detail. [11C]-Dihydrotetrabenazine uptake in the pancreas was demonstrated on micro-PET scanning in the sham and duodenojejunal bypass rodents.CONCLUSION:
Intensive medical management in the perioperative period and attention to the operative technique lowered the mortality. [11C]-Dihydrotetrabenazine micro-PET scanning is a feasible method for assessing the beta-cell mass in GK rodents and could prove to be an important modality for evaluating beta-cell performance in type 2 diabetes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Duodenostomia
/
Jejunostomia
/
Tomografia por Emissão de Pósitrons
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Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
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Células Secretoras de Insulina
/
Gastrectomia
Limite:
Animals
Idioma:
En
Revista:
Surg Obes Relat Dis
Assunto da revista:
METABOLISMO
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos