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Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids.
González-Rioja, Roberto; Asturias, Juan A; Martínez, Alberto; Goñi, Félix M; Viguera, Ana Rosa.
Afiliação
  • González-Rioja R; Research and Development Department, Bial-Arístegui, Bilbao, Spain.
FEBS J ; 276(6): 1762-75, 2009 Mar.
Article em En | MEDLINE | ID: mdl-19236482
ABSTRACT
Par j 1 and Par j 2 proteins are the two major allergens in Parietaria judaica pollen, one of the main causes of allergic diseases in the Mediterranean area. Each of them contains eight cysteine residues organized in a pattern identical to that found in plant nonspecific lipid transfer proteins. The 139- and 102-residue recombinant allergens, corresponding respectively to Par j 1 and Par j 2, refold properly to fully functional forms, whose immunological properties resemble those of the molecules purified from the natural source. Molecular modeling shows that, despite the lack of extensive primary structure homology with nonspecific lipid transfer proteins, both allergens contain a hydrophobic cavity suited to accommodate a lipid ligand. In the present study, we present novel evidence for the formation of complexes of these natural and recombinant proteins from Parietaria pollen with lipidic molecules. The dissociation constant of oleyl-lyso-phosphatidylcholine is 9.1 +/- 1.2 microm for recombinant Par j 1, whereas pyrenedodecanoic acid shows a much higher affinity, with a dissociation constant of approximately 1 microm for both recombinant proteins, as well as for the natural mixture. Lipid binding does not alter the secondary structure content of the protein but is very efficient in protecting disulfide bonds from reduction by dithiothreitol. We show that Par j 1 and Par j 2 not only bind lipids from micellar dispersions, but also are able to extract and transfer negative phospholipids from bilayers.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Alérgenos / Metabolismo dos Lipídeos Idioma: En Revista: FEBS J Assunto da revista: BIOQUIMICA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Alérgenos / Metabolismo dos Lipídeos Idioma: En Revista: FEBS J Assunto da revista: BIOQUIMICA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Espanha