The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress.
EMBO J
; 28(8): 1142-56, 2009 Apr 22.
Article
em En
| MEDLINE
| ID: mdl-19279665
ABSTRACT
The Mre11-Rad50-Xrs2 (MRX) complex has an important function in the maintenance of genomic integrity by contributing to the detection and repair of chromosome breaks. Here we show that the complex is recruited to sites of paused forks where it stabilizes the association of essential replisome components. Interestingly, this function is not dependent on the S phase checkpoint or the nuclease activity of Mre11. We find that disruption of the MRX complex leads to a loss of fork recovery and a failure to properly complete DNA replication when cells are exposed to replication stress. Our data suggest that one critical function of the MRX complex during replication is to promote the cohesion of sister chromatids at paused forks, offering an explanation for why MRX deficiency leads to a loss of cell viability and high levels of chromosome rearrangements under conditions of replication stress.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fase S
/
Proteínas de Saccharomyces cerevisiae
/
Complexos Multiproteicos
/
Proteínas de Ligação a DNA
/
Replicação do DNA
/
Endodesoxirribonucleases
/
Exodesoxirribonucleases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Canadá