[Induction of apoptosis by proteasome inhibitor MG-132 in human erythroleukemia cell line K562].
Zhongguo Dang Dai Er Ke Za Zhi
; 11(4): 255-8, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19374806
ABSTRACT
OBJECTIVE:
To investigate whether proteasome inhibitor MG-132 induces apoptosis of human erythroleukemia cell line K562 and possible mechanisms.METHODS:
K562 cells were incubated with RPMI 1640 and exposed to 0, 1, 5, 10, 15 micromol/L of MG-132 for 24 hrs, respectively. The apoptosis of cells were detected by fluorescence microscope, DNA fragments and flow cytometry. The NF-kappaB mRNA expression was quantified by reverse transcription-polymerase chain reaction (RT-PCR). Expression of NF-kappaB and caspase-3 was semiquantitatively analyzed with SABC techniques. Caspase-3 activities were measured with a colorimetric method.RESULTS:
The growth of K562 cells was inhibited and the apoptosis of the cells increased after MG-132 treatment in a dose-dependent manner. After 24 hrs of 15 micromol/L MG-132 treatment, the percentage of apoptotic cells (26.5+/-0.6%) increased significantly when compared with the untreated controls (1.2+/-0.1%) (P<0.01). MG-132 treatment decreased the mRNA and protein expression of NF-kappaB, and increased the protein expression of caspase-3.CONCLUSIONS:
MG-132 can induce apoptosis of human erythroleukemia cell line K562 through the down-regulation of NF-kappaB expression and up-regulation of caspase-3 expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores de Cisteína Proteinase
/
Apoptose
/
Inibidores de Proteassoma
/
Leupeptinas
/
Antineoplásicos
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Dang Dai Er Ke Za Zhi
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China