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Label-free electrochemical detection of the p53 core domain protein on its antibody immobilized electrode.
Yeo, Jongchan; Park, Jin-Young; Bae, Won Jin; Lee, Yoon Suk; Kim, Byeang Hyean; Cho, Yunje; Park, Su-Moon.
Afiliação
  • Yeo J; Department of Chemistry, Center for Integrated Molecular Systems, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Korea.
Anal Chem ; 81(12): 4770-7, 2009 Jun 15.
Article em En | MEDLINE | ID: mdl-19435284
ABSTRACT
We report quantitative results on interactions between a tumor suppressor protein, p53, also known as a prognostic cancer marker, and its antibody. The p53 antibody molecules immobilized on an (R)-lipo-diaza-18-crown-6 self-assembled monolayer (SAM)-modified gold disk electrode were shown to effectively capture the p53 protein by Western blot, quartz crystal microbalance, and electrochemical impedance experiments. The p53 protein thus captured modulated the ability of the electrode for charge transfer to and from a redox probe in the solution in a p53 concentration range of approximately 0.1-30 microg/mL. The same interaction was also observed in the human embryonic kidney cell lysate, demonstrating that the SAM-modified electrode can serve as a selective platform for electrochemically monitoring the cellular p53 concentration.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Eletroquímica / Eletrodos / Anticorpos Imobilizados Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Eletroquímica / Eletrodos / Anticorpos Imobilizados Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2009 Tipo de documento: Article