Expression artifact with retroviral vectors based on pBMN.
Anal Biochem
; 395(1): 49-53, 2009 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-19602420
ABSTRACT
While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
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Transfecção
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Expressão Gênica
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Éxons
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Artefatos
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Vetores Genéticos
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Vírus da Leucemia Murina de Moloney
Limite:
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos