Site-specific two-color protein labeling for FRET studies using split inteins.
J Am Chem Soc
; 131(33): 11644-5, 2009 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-19645470
ABSTRACT
Fluorescence resonance energy transfer (FRET) is an ideal tool for studying protein conformational changes and dynamics. Strategies to specifically label proteins with multiple colors at structurally important sites, a prerequisite for such studies, however, need to be devised on a protein-by-protein basis. Here we describe a simple yet widely applicable two-color site-specific protein labeling method utilizing Npu DnaE split intein.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Transferência Ressonante de Energia de Fluorescência
/
Inteínas
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Taiwan