Amino acid substitution at position 464 in the haemagglutinin-neuraminidase protein of a mumps virus Urabe strain enhanced the virus growth in neuroblastoma SH-SY5Y cells.
Vaccine
; 27(44): 6160-5, 2009 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-19712764
ABSTRACT
Mumps virus (MuV) infects various organs including central nervous system (CNS). However, the molecular basis of the neural cell specificity of MuV is not well understood. We found that the Hoshino vaccine strain rescued from cDNA replicated moderately in neuroblastoma SH-SY5Y cell line, while an Urabe strain (Ur89-250) isolated from a post-vaccination aseptic meningitis case replicated efficiently in the same cells. In order to examine the contribution of individual genes of Ur89-250 to the growth in SH-SY5Y cells, recombinant Hoshino vaccine strains in which each gene(s) was replaced with corresponding gene(s) of Ur89-250 were generated. A recombinant virus possessing the small hydrophobic and haemagglutinin-neuraminidase (HN) genes of Ur89-250 grew as efficiently in SH-SY5Y cells as Ur89-250. Further analysis indicated that an amino acid substitution at position 464 in the HN protein was most important for efficient growth. Thus, single amino acid substitution in the HN protein could affect neural cell specificity of mumps virus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína HN
/
Substituição de Aminoácidos
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Vírus da Caxumba
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão