LC determination of albiflorin and paeoniflorin in Bai Shao (Paeonia lactiflora) as a raw herb and dried aqueous extract.
J AOAC Int
; 92(4): 1027-34, 2009.
Article
em En
| MEDLINE
| ID: mdl-19714968
ABSTRACT
A validated analytical method is reported for the analysis of paeoniflorin and albiflorin in Bai Shao (Paeonia lactiflora) as a dried raw herb and commercially prepared dried aqueous extract. The samples were extracted by sonication in methanol and the extract analyzed by LC-photodiode array with identity confirmation by electrospray ionization-tandem MS. A C18 column was used with an acetonitrile-water gradient mobile phase. Paeoniflorin and albiflorin were quantified at 230 nm. Ions with m/z 121 and 327 were produced with the MS detector, using the paeoniflorin precursor ion with m/z 479. For albiflorin, the precursor ion with m/z 479 produced the m/z 121 and 77 ions. The amounts of paeoniflorin and albiflorin found in the raw herb were 33.2 and 1.8 mg/g, respectively; and in the dried aqueous extract, the amounts were 34.8 and 15.7 mg/g, respectively. The LODs for paeoniflorin and albiflorin were 0.37 and 1.39 mg/g, respectively, for the raw herb and 0.25 and 0.06 mg/g, respectively, for the dried aqueous extract.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzoatos
/
Hidrocarbonetos Aromáticos com Pontes
/
Paeonia
/
Glucosídeos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J AOAC Int
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Austrália