Effects of growth factors on equine synovial fibroblasts seeded on synthetic scaffolds for avascular meniscal tissue engineering.
Res Vet Sci
; 88(2): 326-32, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-19720387
ABSTRACT
Across species, the avascular portion of the knee meniscus cannot heal spontaneously if severely injured. The most common treatment is meniscectomy which results in osteoarthritis. The objective of this study was to assess the fibrochondrogenic potential of equine fibroblast-like synoviocytes (FLS) seeded on scaffolds under the influence of growth factors in vitro to determine the potential of developing a novel cell-based repair strategy. Cultured FLS were seeded onto synthetic scaffolds in a rotating bioreactor under the influence of three growth factor regimens none, basic fibroblast growth factor (bFGF) alone, and bFGF plus transforming growth factor (TGF-beta(1)) and insulin-like growth factor (IGF-1). Constructs were analyzed for mRNA expression and production of fibrochondroid extracellular matrix constituents. Type II collagen and aggrecan mRNA were significantly higher in growth factor-treated groups (p<0.05). Despite sub-optimal extracellular matrix production, FLS can exhibit fibrochondral characteristics and may have potential for cell-based tissue engineering for avascular meniscal regeneration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Insulin-Like I
/
Fator de Crescimento Transformador beta
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Engenharia Tecidual
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Fatores de Crescimento de Fibroblastos
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Fibroblastos
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Cavalos
Limite:
Animals
Idioma:
En
Revista:
Res Vet Sci
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos