Presence of mitochondrial D-loop DNA in scrapie-infected brain preparations enriched for the prion protein.
J Virol
; 64(7): 3265-8, 1990 Jul.
Article
em En
| MEDLINE
| ID: mdl-1972202
ABSTRACT
The prion preparation has, in recent years, been the focal point of scrapie research. The inability to identify agent-specific nucleic acids in this sample has led to the formulation of the infectious protein or prion hypothesis. In this study, we analyzed three different prion protein-enriched preparations and found all to contain significant amounts of mitochondrial nucleic acid. Southern blot analyses indicated that they are enriched for a specific component of the mitochondrial genome, the single-stranded displacement loop fragment. Our results suggest that if mitochondrial nucleic acids are involved in scrapie infection, it is the displacement loop fragment that is specifically responsible.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Scrapie
/
Proteínas Virais
/
DNA Mitocondrial
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
1990
Tipo de documento:
Article