Determination of intracellular glutathione and cysteine using HPLC with a monolithic column after derivatization with monobromobimane.
Biomed Chromatogr
; 24(5): 455-7, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-19739244
ABSTRACT
An optimized high-performance liquid chromatography (HPLC) method is used to show that, as myoblasts differentiate into multinucleated muscle fibers, there is a shift to a more oxidized cell redox state. The HPLC method incorporated derivatization with monobromobimane for the determination of the reduced (GSH) and oxidized (GSSG) forms of glutathione and the reduced (Cys) and oxidized (CysSS) forms of cysteine. The derivatization was optimized to improve the sensitivity of the approach; the limits of detection for glutathione and cysteine were 3 x 10(-8) and 5 x 10(-8) M, respectively.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Músculo Esquelético
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Dissulfeto de Glutationa
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Cisteína
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Espaço Intracelular
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Glutationa
Tipo de estudo:
Evaluation_studies
Limite:
Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2010
Tipo de documento:
Article