The genetic backbone modulates the phenotype of hepatitis B surface antigen mutants.
J Gen Virol
; 91(Pt 1): 68-73, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19759242
ABSTRACT
Hepatitis B virus (HBV) vaccine and diagnostic escape mutants are a growing concern. The principle target of detection, hepatitis B surface antigen (HBsAg), encoded by S, is completely overlapped by the reverse transcriptase encoding P. With the increased incidence of nucleos(t)ide analogue resistance altering P, the concurrent impact on S must be assessed. HBV DNA from 59 HBsAg-positive plasma samples was sequenced across the polymerase/surface region and the amino acid sequence of HBsAg was inferred. ELISAs were formatted containing individually bound monoclonal antibodies directed against three discrete epitopes on HBsAg. Similar point mutations occurring in different genotypes were shown to influence epitope conformation differently, indicating that the genetic backbone is a major factor in predicting phenotype. C-terminal changes associated with antiviral resistance were found to modulate epitope profiles of HBsAg. Treatment options which may promote drug resistance should be avoided to both protect antiviral treatment and prevent facilitation of vaccine and diagnostic escape mutants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Hepatite B
/
Mutação de Sentido Incorreto
/
Farmacorresistência Viral
/
Antígenos de Superfície da Hepatite B
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido