Localization of Na+-K+ ATPases in quasi-native cell membranes.
Nano Lett
; 9(12): 4489-93, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19807066
ABSTRACT
Na(+)-K(+) ATPases have been observed and located by in situ AFM and single molecule recognition technique, topography and recognition imaging (TREC) that is a unique technique to specifically identify single protein in complex during AFM imaging. Na(+)-K(+) ATPases were well distributed in the inner leaflet of cell membranes with about 10% aggregations in total recognized proteins. The height of Na(+)-K(+) ATPases measured by AFM is in the range of 12-14 nm, which is very consistent with the cryoelectron microscopy result. The unbinding force between Na(+)-K(+) ATPases in the membrane and anti-ATPases on the AFM tip is about 80 pN with the apparent loading rate at 40 nN/s. Our results show the first visualization of an essential membrane protein, Na(+)-K(+) ATPase, in quasi-native cell membranes and may be significant to reveal the interactions between Na(+)-K(+) ATPases and other membrane proteins at the molecular level.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Sonda Molecular
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ATPase Trocadora de Sódio-Potássio
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Microscopia de Força Atômica
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Materiais Biomiméticos
/
Membranas Artificiais
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2009
Tipo de documento:
Article