Bacterial diversity in surgical site infections: not just aerobic cocci any more.
J Wound Care
; 18(8): 317-23, 2009 Aug.
Article
em En
| MEDLINE
| ID: mdl-19862869
ABSTRACT
OBJECTIVE:
To evaluate the microbial diversity in chronic surgical site infections (SSIs).METHOD:
Bacterial populations in 23 chronic SSIs were identified using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP),which is an universal bacterial identification method.These results were then validated using quantitative polymerase chain reaction (qPCR).RESULTS:
bTEFAP identified two previously uncharacterised Bacteroidales in all of the SSIs and showed that it was the predominant population in the majority of these chronic wounds. Other bacteria identified included Corynebacterium spp., Peptoniphilus spp., Staphylococcus spp., Staphylococcus aureus, Serratia marcescens, Prevotella spp. and Pseudomonas aeruginosa. Rarefaction analysis of the data indicated that, on average, six genera occurred in any given SSI, suggesting that such infections are multispecies. On average, over 60% of the bacteria evaluated in the SSIs were anaerobic bacilli. The previous literature indicates that aerobic cocci predominate in such wounds.CONCLUSION:
This modern molecular survey indicates that our previous understanding of which bacteria cause SSIs may be faulty. The high prevalence of anaerobic bacilli and the overwhelming predominance of two previously uncharacterised Bacteroidales suggest that such bacteria may be a leading contributor to such infections. Further research on the identification and treatment of such bacteria are warranted.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecção da Ferida Cirúrgica
/
Infecções Bacterianas
Tipo de estudo:
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Wound Care
Assunto da revista:
ENFERMAGEM
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos