Biofilm formation by Pseudomonas aeruginosa on ossicular reconstruction prostheses.
Am J Otolaryngol
; 30(6): 367-70, 2009.
Article
em En
| MEDLINE
| ID: mdl-19880023
ABSTRACT
PURPOSE:
Ossicular chain reconstruction may be complicated by prosthesis extrusion. As prostheses are commonly placed in middle ears contaminated with biofilm-forming bacteria, such as Pseudomonas aeruginosa (PA), extrusion may be caused by development of a biofilm on the prosthesis and the host response to this biofilm. The purpose of this experiment was to determine if PA forms biofilm on different ossicular chain reconstruction prostheses to a different degree.METHODS:
Prostheses made of titanium, hydroxylapatite (HA), and plastic (23 each) were cultured with PA in broth for 96 hours. Biofilm formation was assessed by electron microscopy and quantitative microbiology.RESULTS:
Titanium prostheses formed less biofilm than plastic (P = .0003) and HA (P = .003), but there was no difference between HA and plastic. Correction for surface area did not alter these significant differences.CONCLUSIONS:
Pseudomonas aeruginosa forms biofilm on ossicular prostheses, particularly those made of plastic and HA. These differences could, in part, explain the extrusion propensity of certain biomaterials.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Infecções por Pseudomonas
/
Prótese Ossicular
/
Infecções Relacionadas à Prótese
/
Biofilmes
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Revista:
Am J Otolaryngol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos