Evaluation of the genotoxic and anti-genotoxic activities of silybin in human hepatoma cells (HepG2).
Mutagenesis
; 25(3): 223-9, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20032005
ABSTRACT
Silybin (SB), a constituent of the medicinal plant Silybum marianum, is reported to be a potent hepatoprotective agent, but little is currently known regarding its genotoxicity, mutagenicity and potential chemopreventive properties. In this study, we evaluated the ability of SB to induce DNA migration and micronuclei (MN) formation in human hepatoma cells (HepG2). Also, possible preventive effects of SB on MN formation induced by three different mutagens, bleomycin (BLEO), benzo[a]pyrene (B[a]P) and aflatoxin B(1) (AFB(1)), were studied. To clarify the possible mechanism of SB antimutagenicity, three treatment protocols were applied pretreatment, in which SB was added before the application of the mutagens; simultaneous treatment, in which SB was added during treatment and post-treatment, in which SB was added after the application of the mutagens. At concentrations up to 100 microM, SB was non-genotoxic, while at a concentration of 200 microM, SB induced DNA migration, generated oxidized DNA bases, reduced cell viability, decreased the replicative index of the cells and induced oxidative stress. It is noteworthy that SB was able to reduce the genotoxic effect induced by B[a]P, BLEO and AFB(1) in pretreatment and simultaneous treatments but had no significant effect on DNA damage induction in post-treatment. Taken together, our findings indicate that SB presents anti-genotoxic activity in vitro, which suggests potential use as a chemopreventive agent.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Silimarina
/
Carcinoma Hepatocelular
/
Neoplasias Hepáticas
/
Mutagênicos
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Mutagenesis
Assunto da revista:
GENETICA MEDICA
/
SAUDE AMBIENTAL
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Brasil