[Anti-tumor compounds from submerger fermentation products of a Paecilomyces militaris strain].
Wei Sheng Wu Xue Bao
; 49(10): 1332-40, 2009 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-20069879
ABSTRACT
OBJECTIVE:
The aim was to identify the antitumor compounds of the fermentation broth of Paecilomyces militaris (strain RCEF0927).METHODS:
Anti-tumor activity was tested with a resazurin cytotoxicity assay model with Chinese hamster ovary (CHO) cells. Bioactive compounds were analysed with combined method of High Performance Liquid Chromatography (HPLC), High Resolution Mass Spectrometer (HRMS) and antitumor assay.RESULTS:
Antitumor test showed that the fermentation broth had strong cytotoxicity against CHO cells. Extraction tests showed that ethylacetate was the best solvent for the bioactive constituents extracting. HPLC-DAD- HRMS-Cytotoxicity assay revealed that the molecular formula of the active compounds in the extract were possibly C10 H13 N5 O3, C22 H22 O9, C23 H24 O10, C22 H22 O10, C15 H10 O4, C15 H10O, C36 H70 O11, C36 H68 O12, C38 H74 O11, C40 H76 O13 and C44 H82 O14.CONCLUSION:
The possible structures of the bioactive compounds were deduced with bioassay, HRMS, Uv and database inquiry as cordycepin, daizein, genistein 7,4'-dihydroxyisoflavone-7-O-(4"-O-methyl)-b-D-glucopyranoside, 7, 4'-dihydroxy-6- methoxyisoflavone-7-O-( 4"-O-methyl)-b-D-glucopyranoside, 5, 7, 4'-trihydroxy-isoflavone-7-O-(4"-O-methyl)-b-D-glucopyranoside, polyoxyethylene ether oleate, dehydro products of polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxypropylene or polyoxybutene contained derivants of polyoxyethylene sorbitan fatty acid ester. Cytotoxicity of the compounds was revealed for the first time.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paecilomyces
/
Técnicas de Cultura
/
Fermentação
/
Antineoplásicos
Limite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Wu Xue Bao
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China