[Regulative role of TNFalpha on STEAP4 gene in matured human adipocytes].
Zhongguo Dang Dai Er Ke Za Zhi
; 11(12): 1008-11, 2009 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-20113612
ABSTRACT
OBJECTIVE:
Human STEAP4, a novel obesity-related gene, is associated with insulin sensitivity regulation in human adipocytes. This study aimed to explore the regulative role of TNFalpha on STEAP4 gene in matured human adipocytes.METHODS:
Human preadipocytes were cultured and differentiated into matured adipocytes in vitro. Fully differentiated adipocytes (Day 17) were treated with different concentrations of TNFalpha (0, 5, 10, 25 and 50 ng/mL) for 24 hrs. Total RNA and protein were extracted from the adipocytes. Levels of STEAP4 mRNA and protein expression were determined by real-time quantitative RT-PCR and Western blot respectively.RESULTS:
Different concentrations (5, 10, 25 and 50 ng/mL) of TNFalpha treatment for 24 hrs resulted in a significant increase in the STEAP4 mRNA expression of human matured adipocytes.The maximal effect was seen in the 50 ng/mL of TNFalpha treatment group. In parallel, STEAP4 protein synthesis in matured adipocytes increased in response to TNFalpha treatment of different concentrations (5, 10, 25 and 50 ng/mL) for 24 hrs. The maximal up-regulated effect was seen in the 25 ng/mL of TNFalpha treatment group.CONCLUSIONS:
TNFalpha can up-regulate STEAP4 mRNA expression in human matured adipocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Fator de Necrose Tumoral alfa
/
Adipócitos
/
Proteínas de Membrana
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Dang Dai Er Ke Za Zhi
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China