Purification and crystallization of Phd, the antitoxin of the phd/doc operon.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 66(Pt 2): 167-71, 2010 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-20124714
ABSTRACT
The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óperon
/
Proteínas Virais
/
Antitoxinas
/
Bacteriófago P1
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Bélgica