GC-MS for characterization and identification of ant semiochemicals.
Cold Spring Harb Protoc
; 2009(7): pdb.prot5249, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-20147214
ABSTRACT
Living in a predominantly dark environment, ants rely mostly on chemical signals for communication. Trail and alarm pheromones are the most widely studied and best characterized of all ant semiochemicals, but other such compounds can influence a variety of other behaviors, including reproductive activities, sexual development, nest mate and caste recognition, and defense. A typical worker body contains more than 10 different semiochemical-producing glands, and the surface of the cuticle is covered with lipids that serve as recognition signals. The methods of choice for collection and identification of ant semiochemicals should be determined based on results of behavioral analyses. These can indicate the source (e.g., glandular, cuticular) and the nature (volatile vs. nonvolatile) of the chemical. This protocol presents a number of different methods for collecting lipid semiochemicals. These can be followed by gas chromatography (GC) coupled with mass spectrometry (MS) to better characterize, and possibly identify, the semiochemical in question.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Formigas
/
Feromônios
/
Biologia
/
Cromatografia Gasosa-Espectrometria de Massas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos