Assay restriction profiles of three monoclonal antibodies recognizing the G3m(u) allotype. Development of an allotype specific assay.
J Immunol Methods
; 138(1): 57-64, 1991 Apr 08.
Article
em En
| MEDLINE
| ID: mdl-2019747
ABSTRACT
Three monoclonal antibodies raised against a purified human IgG3 paraprotein were found to exhibit a restriction profile for IgG3/G3m(u) and pan-IgG specificity which was dependent on the assay system. When adapted to an IgG3 subclass capture ELISA, all three McAbs discriminated between paraproteins expressing G3m(u) and antithetical markers G3m(st). One of the antibodies (PNF69C) was selected and conditions were optimised for Gm typing purposes. Using this system G3m(u) could be detected on captured IgG3 derived from human sera. This system may prove useful in the elucidation of Gm allotype profiles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alótipos Gm de Imunoglobulina
/
Imunoglobulina G
/
Anticorpos Monoclonais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido