Dose-dependent changes in cyclin D1 in response to 4-nitroquinoline 1-oxide-induced DNA damage.
Arch Biochem Biophys
; 497(1-2): 55-61, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20214871
ABSTRACT
Cells respond to DNA damage by either repairing the damage or committing to a death or senescence pathway, dependent on the level of damage sustained. In this study, we show that the protein levels of cyclin D1 and the CDK inhibitor, p21(CIP1), respond in a dose-dependent manner to the DNA damaging agent, 4-nitroquinoline 1-oxide (4NQO). Cyclin D1 responses were independent of p53 and resulted in a partial loss of Retinoblastoma protein phosphorylation. The differential responses of cyclin D1 and p21(CIP1) were associated with distinct cellular responses in low dose treatments the cells recovered after a lag period whilst at medium and high doses, the cells died through seemingly distinct mechanisms. Our data suggest that the balance between cyclin D1 and p21(CIP1) following exposure to DNA damage may play a key role in determining the subsequent cellular responses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
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Ciclina D1
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Inibidor de Quinase Dependente de Ciclina p21
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Mutagênicos
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4-Nitroquinolina-1-Óxido
Limite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido