An unexpectedly efficient catalytic antibody operating by ping-pong and induced fit mechanisms.
Science
; 252(5006): 680-5, 1991 May 03.
Article
em En
| MEDLINE
| ID: mdl-2024120
ABSTRACT
A transition state analogue was used to produce a mouse antibody that catalyzes transesterification in water. The antibody behaves as a highly efficient catalyst with a covalent intermediate and the characteristic of induced fit. While some features of the catalytic pathway were programmed when the hapten was designed and reflect favorable substrate-antibody interactions, other features are a manifestation of the chemical potential of antibody diversity. The fact that antibodies recapitulate mechanisms and pathways previously thought to be a characteristic of highly evolved enzymes suggests that once an appropriate binding cavity is achieved, reaction pathways commensurate with the intrinsic chemical potential of proteins ensue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catálise
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1991
Tipo de documento:
Article