Procedure for selecting monoclonal antibodies for use in a ligand displacement assay of serum antibody levels.
J Immunoassay
; 12(1): 125-44, 1991.
Article
em En
| MEDLINE
| ID: mdl-2040708
ABSTRACT
Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement format and those which were not.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Anticorpos
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Immunoassay
Ano de publicação:
1991
Tipo de documento:
Article