Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells.
Mem Inst Oswaldo Cruz
; 105(2): 132-6, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20428670
ABSTRACT
In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rhodnius
/
Glândulas Salivares
Limite:
Animals
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Brasil