[Effects of Selaginella doederleinii on human nasopharyngeal carcinoma TW03 cells in vitro and its mechanism].

ABSTRACT

OBJECTIVE: To study the inhibition and mechanism of Selaginella doederleinii (SLG) on the growth of nasopharyngeal carcinoma (NPC) TW03 cells in vitro. METHODS: Cell suppression rates were measured by MTT assay, cell cycles were measured by flow cytometry (FCM) and expressions of Bc1-2 and Bax protein were detected by immunohistochemistry. RESULTS: Inhibition on the growth of NPC cell was intensed by SLG in a dose and time dependent manner in vitro (P < 0.05). The ratio of S phase cells increased and the ratios of G0/G1 and G2/M phase cells decreased, with the addition of SLG. Meanwhile, the expression of Bcl-2 protein was down-regulated and Bax protein was up-regulated. CONCLUSION: SLG could inhibit the growth of human NPC TW03 cells by directing cell cycle arrested at S phase, down-regulating Bcl-2 protein expression and up-regulating Bax protein expression.