Covalent monofunctionalization of peptide-coated quantum dots for single-molecule assays.
Nano Lett
; 10(6): 2147-54, 2010 Jun 09.
Article
em En
| MEDLINE
| ID: mdl-20433164
ABSTRACT
Fluorescent probes for biological imaging of single molecules (SM) have many stringent design requirements. In the case of quantum dot (QD) probes, it remains a challenge to control their functional properties with high precision. Here, we describe the simple preparation of QDs with reduced size and monovalency. Our approach combines a peptide surface coating, stable covalent conjugation of targeting units and purification by gel electrophoresis. We precisely characterize these probes by ensemble and SM techniques and apply them to tracking individual proteins in living cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Pontos Quânticos
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
França