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Glutamine synthetase as an early marker for hepatocellular carcinoma based on proteomic analysis of resected small hepatocellular carcinomas.
Long, Jiang; Lang, Zhen-Wei; Wang, Hua-Guang; Wang, Tai-Ling; Wang, Bao-En; Liu, Si-Qi.
Afiliação
  • Long J; Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Hepatobiliary Pancreat Dis Int ; 9(3): 296-305, 2010 Jun.
Article em En | MEDLINE | ID: mdl-20525558
BACKGROUND: Hepatocellular carcinoma (HCC) is a highly malignant tumor with a poor prognosis. Because small HCCs possess most of the characteristics of early HCC, we investigated small HCCs to screen potential biomarkers for early diagnosis. METHODS: Proteins were extracted from 10 sets of paired tissue samples from HBV-infected small-HCC patients. The extracted proteins were well resolved by two-dimensional electrophoresis. These HCC-associated proteins were then identified by MALDI-TOF/TOF MS following image analysis. Western blotting and immunohistochemistry were used to assess glutamine synthetase (GS) and phenazine biosynthesis-like domain-containing protein (PBLD) expression in liver tissue. Enzyme-linked immunosorbent assays in 152 serum samples (from 49 healthy donors, 24 patients with liver cirrhosis, and 79 with HCC) were used to further assess the significance of GS clinically. RESULTS: Fifteen up-regulated and three down-regulated proteins were identified. Western blotting confirmed GS overexpression and decreased PBLD expression in liver tissue. Immunohistochemistry showed that GS was expressed in 70.0% (84/120) of HCCs and 35.8% (43/120) of nontumor tissues; PBLD was expressed in 74.2% (89/120) of nontumor tissues and 40.8% (49/120) of HCCs. The Chi-square test showed significant expression differences between HCCs and adjacent tissues. Consistent with this, serum GS levels in HCC patients were significantly higher than those in liver cirrhosis patients and healthy donors, while the latter two groups were also significantly different. In addition, a diagnostic cutoff value of 2.6 mg/ml was used for GS; it was elevated in 19 (76.0%) of 25 HCC patients with AFP
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Hepatocelular / Proteômica / Glutamato-Amônia Ligase / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies / Screening_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Hepatobiliary Pancreat Dis Int Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Hepatocelular / Proteômica / Glutamato-Amônia Ligase / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies / Screening_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Hepatobiliary Pancreat Dis Int Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China