Synchrotron X-ray imaging for nondestructive monitoring of sap flow dynamics through xylem vessel elements in rice leaves.

ABSTRACT

A comprehensive understanding of the sap flow dynamics and xylem hydraulic properties is essential to unravel the functional features of water transport from roots to shoots in vascular plants. To evaluate quantitatively the safety and efficiency of this system, nondestructive methods to assess the interactions between sap ascent kinetics and xylem structure are required. In this study, synchrotron X-ray microscopy was employed to observe anatomical structures and sap flow dynamics in rice (Oryza sativa) xylem simultaneously. The phase-contrast imaging technique allowed nondestructive observation of the xylem structural characteristics and the air-water interfaces generated by dehydration-rehydration cycles in excised leaves. This X-ray microimaging method provided a unique tool to characterize the perforated end walls of vessel elements and to evaluate their influence on hydraulic resistance during the refilling of embolized vessels. The real-time monitoring of the axial and radial sap flow under various environmental conditions highlighted the important role of perforation plates. In summary, we report a new methodology to study the sap flow dynamics and xylem hydraulic properties with µm spatial and ms temporal resolution using X-ray microscopy. The experimental procedure described herein provides a useful handle to understand key sap transport phenomena in xylem.