Your browser doesn't support javascript.
loading
Dimeric character of a basic phospholipase A2 from cobra venom: experimental and modelling study.
Demaret, J P; Chwetzoff, S; Brunie, S.
Afiliação
  • Demaret JP; Laboratoire de Biochimie (CNRS UA 240), Ecole Polytechnique, Palaiseau, France.
Protein Eng ; 4(2): 171-6, 1990 Dec.
Article em En | MEDLINE | ID: mdl-2075192
When it is gel filtered on Sephadex in the absence of calcium ions, basic phospholipase A2 from Naja nigricollis venom elutes as a dimer. In order to study the possibility of this dimerization from a structural point of view, three-dimensional models of both monomeric and dimeric N. nigricollis phospholipases A2 have been graphically built on the basis of homologies with the phospholipases A2 from pancreatic bovine and Crotalus atrox venom. The building of a dimeric model is made possible by the deletion of a particular loop of the bovine structure. The predicted models of N. nigricollis phospholipase A2 have been checked using molecular mechanics and molecular dynamics techniques according to a suitable protocol which has been developed starting from refined X-ray structures of phospholipases A2 as the test case. The observed stability of the dimeric model, in the absence of calcium, agrees with the hypothesis of the dimerization of the basic phospholipase A2. Particularly, Arg31, which replaces the hydrophobic residue present in pancreatic bovine and C.atrox venom phospholipases A2, contributes to this stability.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfolipases A / Modelos Moleculares Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Protein Eng Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 1990 Tipo de documento: Article País de afiliação: França
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfolipases A / Modelos Moleculares Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Protein Eng Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 1990 Tipo de documento: Article País de afiliação: França