Nonequilibrium energetics of a single F1-ATPase molecule.
Phys Rev Lett
; 104(19): 198103, 2010 May 14.
Article
em En
| MEDLINE
| ID: mdl-20867002
ABSTRACT
Molecular motors drive mechanical motions utilizing the free energy liberated from chemical reactions such as ATP hydrolysis. Although it is essential to know the efficiency of this free energy transduction, it has been a challenge due to the system's microscopic scale. Here, we evaluate the single-molecule energetics of a rotary molecular motor, F1-ATPase, by applying a recently derived nonequilibrium equality together with an electrorotation method. We show that the sum of the heat flow through the probe's rotational degree of freedom and the work against an external load is almost equal to the free energy change per a single ATP hydrolysis under various conditions. This implies that F1-ATPase works at an efficiency of nearly 100% in a thermally fluctuating environment.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
ATPases Translocadoras de Prótons
/
Ensaios Enzimáticos
Idioma:
En
Revista:
Phys Rev Lett
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão