Activation of the pacidamycin PacL adenylation domain by MbtH-like proteins.
Biochemistry
; 49(46): 9946-7, 2010 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-20964365
ABSTRACT
Nonribosomal peptide synthetase (NRPS) assembly lines are major avenues for the biosynthesis of a vast array of peptidyl natural products. Several hundred bacterial NRPS gene clusters contain a small (â¼70-residue) protein belonging to the MbtH family for which no function has been defined. Here we show that two strictly conserved Trp residues in MbtH-like proteins contribute to stimulation of amino acid adenylation in some NRPS modules. We also demonstrate that adenylation can be stimulated not only by cognate MbtH-like proteins but also by homologues from disparate natural product pathways.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Proteínas de Bactérias
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos